Enzymatic DNA Synthesis

With better tools comes better understanding



Our disruptive Enzymatic Synthesis Technology is transforming how molecular biology contributes to human health, diagnostics, and personalized medicine.
how it works
technology innovations

Synthesis Enzymes

DNA Script has developed and optimized a portfolio of proprietary synthesis enzymes engineered to perform de novo synthesis of nucleic acids with high fidelity and high coupling efficiency.


Reversibly Terminated Nucleotides

DNA Script has developed a range of modified nucleotides containing a reversible terminator on the 3’ position to enable the incorporation of just one nucleotide per addition cycle.


Surface Chemistry

DNA Script uses a surface chemistry with two key functions: a
solid support and predefined initiator DNA (iDNA) linkers. The
physical properties and chemical properties of the solid support are optimized to enable the synthesis of full-length DNA to support molecular biology and genomics applications and provide a surface to which the iDNA is bound. The iDNA contains an enzymatically cleavable nucleotide and uniquely can be customized to incorporate a conserved sequence into each oligo synthesized.

The 2-Step Enzymatic Synthesis Revolution

Oligos are enzymatically synthesized in a cyclic, 2-step process:

Step 1. Elongate: The TdT enzyme adds a single nucleotide to the iDNA.
Step 2. Deprotect: The reversible terminator of the nucleotide is removed, leaving the strand ready to be elongated again.
Steps 1 and 2 are repeated until the longest oligo on the plate is completed.

Following completion of the last synthesis cycle, enzymatic cleavage is performed to release oligo sequences downstream from the cleavage site. The resulting oligos are desalted, quantified, and normalized. Molecular biology-ready oligos are collected, immediately ready for the next step of the molecular biology workflow, and the SYNTAX System may be prepared for another run within just 30 minutes.

DNA as

Nature

Intended

advantages of EDS
EDS DNA by Syntax System Chemically Synthesized DNA as a Service
Enzymatic DNA Synthesis
Biology-based synthesis.
No organic solvents to handle.
Chemical Synthesis
Phosphoramidite chemistry developed over 40 years ago.
May use harsh chemicals.
Simple
Integrated experience.
Accessible to any lab.
Complex
Transactions often involve multiple touchpoints.
Supply logistics may be complex.
Certain
Guaranteed delivery of oligos.
No logistical or production queue issues.
Uncertain
Turnaround times may be inconsistent and unreliable.
Can’t rely on service for critical supply.
Fast
Same-day results, from start to finish, are now accessible.
Run overnight for increased efficiencies.
Slow
Slower turnaround may limit the number of experiments performed each day.
Slower turnaround may limit your ability to respond quickly.
Secure
DNA is not contaminated by other customer projects.
Information can remain confidential behind your firewall.
Vulnerable
Centralized processing may increase risk of contamination.
Proprietary sequencing information is shared with the oligo manufacturer.