Enzymatic DNA Synthesis
With better tools comes better understanding
Our disruptive Enzymatic Synthesis Technology is transforming how molecular biology contributes to human health, diagnostics, and personalized medicine.
how it works
DNA Script has developed and optimized a portfolio of proprietary synthesis enzymes engineered to perform de novo synthesis of nucleic acids with high ﬁdelity and high coupling efficiency.
Reversibly Terminated Nucleotides
DNA Script has developed a range of modified nucleotides containing a reversible terminator on the 3’ position to enable the incorporation of just one nucleotide per addition cycle.
DNA Script uses a surface chemistry with two key functions: a
solid support and predefined initiator DNA (iDNA) linkers. The
physical properties and chemical properties of the solid support are optimized to enable the synthesis of full-length DNA to support molecular biology and genomics applications and provide a surface to which the iDNA is bound. The iDNA contains an enzymatically cleavable nucleotide and uniquely can be customized to incorporate a conserved sequence into each oligo synthesized.
The 2-Step Enzymatic Synthesis Revolution
Oligos are enzymatically synthesized in a cyclic, 2-step process:
Step 1. Elongate: The TdT enzyme adds a single nucleotide to the iDNA.
Step 2. Deprotect: The reversible terminator of the nucleotide is removed, leaving the strand ready to be elongated again.
Steps 1 and 2 are repeated until the longest oligo on the plate is completed.
Following completion of the last synthesis cycle, enzymatic cleavage is performed to release oligo sequences downstream from the cleavage site. The resulting oligos are desalted, quantiﬁed, and normalized. Molecular biology-ready oligos are collected, immediately ready for the next step of the molecular biology workflow, and the SYNTAX System may be prepared for another run within just 30 minutes.
advantages of EDS
|EDS DNA by Syntax System||Chemically Synthesized DNA as a Service|
Enzymatic DNA Synthesis
No organic solvents to handle.
Phosphoramidite chemistry developed over 40 years ago.
May use harsh chemicals.
Accessible to any lab.
Transactions often involve multiple touchpoints.
Supply logistics may be complex.
Guaranteed delivery of oligos.
No logistical or production queue issues.
Turnaround times may be inconsistent and unreliable.
Can’t rely on service for critical supply.
Same-day results, from start to finish, are now accessible.
Run overnight for increased efficiencies.
Slower turnaround may limit the number of experiments performed each day.
Slower turnaround may limit your ability to respond quickly.
DNA is not contaminated by other customer projects.
Information can remain confidential behind your firewall.
Centralized processing may increase risk of contamination.
Proprietary sequencing information is shared with the oligo manufacturer.