Iterate Faster: Design-Print-Test-Learn
More than ever before, science and innovation are intersecting in a race for results, whether it is developing new cell & gene therapies, mRNA personalized vaccines, immune system modular drugs, or new diagnostics tests, researchers need to get there faster. The SYNTAX STX-200 Platform has a small footprint that enables users to enzymatically print DNA, in the laboratory, for a wide range of genomics and synthetic biology applications to accelerate their development workflows.
The speed of the SYNTAX Platform and the breadth of applications enabled by the reagent kits allow genomics researchers who need DNA oligos, primers, and probes for real-time qPCR, Sanger sequencing, next-generation sequencing (NGS), synthetic biology (CRISPR, gene synthesis, and mutagenesis), FISH (fluorescence in situ hybridization), and smFISH applications to finish their experiments before others even start.
With a single benchtop SYNTAX Platform and an expanding line of reagent kits for various applications, users can continue to eliminate bottlenecks to rapidly iterate their experiments by replacing oligos ordered from commercial suppliers with oligos printed in-house, on-demand. Download the Oligo Benchmarking Study here: https://dev-dnascript.pantheonsite.io/enzymatic-dna-synthesis/.
SYNTAX 96 Hi-Fidelity Kits
The SYNTAX 96 Hi-Fidelity Kits include the reagent and consumables required for the parallel enzymatic synthesis of 96 ready-to-use DNA oligos of up to 120 nucleotides (nt) in length and are intended for use in applications requiring high sequence accuracies, such as gene assembly, protein mutagenesis, or CRISPR gene editing.
- Turnaround time: ~6 hours for 20mer oligos; ~23 hours for 120mer oligos
- Oligo length: 15-120nt
- Yield and concentration: ≥200 pmol and ≥5 μM (≥95% of wells)*
*For oligos determined to be of standard synthesis difficulty by the Console Software
SYNTAX 96 Standard Kits
The SYNTAX 96 Standard Kits include all reagents and consumables required for the parallel enzymatic synthesis of 96 ready-to-use DNA oligos of up to 80 nt in length and are intended for use in applications requiring labelled oligos, those requiring higher yield of oligos, or with degenerate bases.
- Turnaround time: ~6 hours for 20mer oligos; ~17 hours for 80mer oligos
- Oligo length: 15-80nt
- Yield and concentration: ≥550 pmol and ≥7 μM (>95% of wells)*
- Yield and concentration (labeled oligos): ≥280 pmol and ≥4 μM (>95% of wells)*
*For oligos determined to be of standard synthesis difficulty by the Console Software
SYNTAX 192 Label Kit
The SYNTAX 192 Label Kit includes supplemental reagents and consumables required for the parallel enzymatic synthesis of ready-to-use labelled DNA probes on a SYNTAX System. Users can synthesize 96 single-labelled probes, e.g., biotinylated probes, or dual-labelled probes, e.g., with a fluorophore and quencher. Labelled probes are rapidly synthesized in less than a day, enabling same or next-day use in downstream workflows. Probe synthesis requires the use of the SYNTAX DNA Synthesis kit, the SYNTAX 96 Standard run kit, and SYNTAX Labels (sold separately).
- Use labelled probes for a variety of techniques, including qPCR, NGS target enrichment, and FISH.
- Select from a menu of SYNTAX Labels that includes five fluorophores, three quenchers, or biotin. Up to two labels can be incorporated in each probe (each sold separately).
- Select the label of your choice. Fluorophores and quenchers are available in pack sizes of 16, 48, and 96 reactions. Biotin is available in a pack of 96 reactions only.
Select the correct kits to synthesize oligos for the Application of your choice
| SYNTAX 96 Hi-Fidelity Kits | SYNTAX 96 Standard Kits | |
|---|---|---|
| APPLICATION | Gene assembly, protein mutagenesis | qPCR probes, PCR primers, Sanger Sequencing primers, FISH probes |
| OLIGO LENGTH | 15-120 nt | 15-80 nt |
| PROBE SYNTHESIS | Not supported currently | Single or dual labelled probes (fluorophores, quenchers, or biotin) |
| DEGENERATE BASES | Yes | Yes |
| KIT BUNDLE (REQUIRED) | 1) SYNTAX DNA Synthesis Kit 2) SYNTAX 96 Hi-Fidelity Run Kit Or 1) SYNTAX DNA Synthesis Kit 2) Requires the SYNTAX 96 Hi-Fidelity Run Kit, Custom iDNA, Pack of 8 | 1)SYNTAX DNA Synthesis Kit 2) SYNTAX 96 Standard Run Kit With labelled probes: 3)SYNTAX 192 Label Kit Fluorophores/Quenchers/Biotin Or 1) SYNTAX DNA Synthesis Kit 2) Requires the SYNTAX Standard Run Kit, Custom iDNA, Pack of 8 With labeled probes: 3)SYNTAX 192 Label Kit Fluorophores/Quenchers/Biotin |
Select the correct label for your research
SYNTAX Labels include five fluorophores and three quenchers, and users can select the fluorophore and the associated quencher, if required, based on their choice and experimental needs.
| Fluorophore | Peak Excitation | Peak Emission | Quencher | |
|---|---|---|---|---|
| Green (488) | 495 nm | 519 nm | Quencher 1 | Use with FAM channel |
| Yellow (532) | 532 nm | 552 nm | Quencher 1 | Use with HEX channel |
| Orange (550) | 554 nm | 576 nm | Quencher 2 | Use with TAMRA channel |
| Red (565) | 564 nm | 590 nm | Quencher 2 | Use with ROX channel |
| Deep Red (647) | 646 nm | 664 nm | Quencher 3 | Use with CY5 channel |
Primers and probes printed on the SYNTAX System in hours perform comparably to those ordered from service providers.
| Catalog Number | Product Description |
|---|---|
| 101867 | SYNTAX DNA Synthesis Kit |
| 101868 | SYNTAX 96 Hi-Fidelity Run Kit |
| 101870 | SYNTAX 96 Hi-Fidelity Run Kit, Custom iDNA, pack of 8 |
| 101869 | SYNTAX 96 Standard Run Kit |
| 101871 | SYNTAX 96 Standard Run Kit, Custom iDNA, pack of 8 |