Design-Print-Test-Learn, Fast
More than ever before, science and innovation are in a race for results, whether it is an upcoming scientific publication, a new diagnostic test or even a vaccine. Researchers need to get there faster, and the SYNTAX Kits enable users to print DNA for a wide range of genomics and synthetic biology applications and accelerate their development workflows.
The speed of the SYNTAX System and the breadth of applications enabled by the SYNTAX synthesis kits allow genomics researchers who need DNA oligos, primers, and probes for real-time qPCR, digital PCR, Sanger sequencing and next-gen sequencing (NGS), synthetic biology (CRISPR, gene synthesis, and mutagenesis), and FISH and smFISH applications to be done with their experiments before others can start.
An Expanding List of Applications
With a single benchtop SYNTAX System and an expanding line of kits for various applications, users can continue to eliminate bottlenecks and rapidly iterate through their experiments by replacing oligos ordered from commercial suppliers with oligos printed in-house.
- Print oligos up to 80 nt in length
- Synthesize hydrolysis probes and primers in the same run, in as little as 8 hours
- Print oligos with a yield of at least 600 pmol and concentration of at least 7 µM
- Label probes with fluorophores, quenchers, or biotin
SYNTAX 60-Cycle Kit, 96 Oligos
SYNTAX 80-Cycle Kit, 96 Oligos
SYNTAX 192 Label Kit
Print 96 x 60 nt oligos in 13 hours
Print 96 x 80 nt oligos with or without labels
Label oligos for various applications
Use for applications that require unlabeled oligos printed in a few hours
Use for applications that require labeled oligos or extra-long oligos
Many labels available for a wide range of applications
- End Point PCR
- SYBR Green qPCR
- Sanger Sequencing
- Gene Assembly
- Real-time qPCR
- Genotyping
- dPCR
- smFISH
- Targeted NGS
- Fluorophores
- Quenchers
- Biotin
SYNTAX 60-Cycle Kit, 96 Oligos
SYNTAX 60-Cycle Kit, 96 Oligos includes the reagents and consumables required for the parallel enzymatic synthesis of 96 ready-to-use DNA oligos of up to 60 nt in length. Oligos are synthesized rapidly to enable same or next day use in downstream workflows.
- Flexible runs: Perform up to two runs per kit using the SYNTAX System to synthesize up to 96 DNA oligos of ≤60 nt or 192 oligos of ≤30 nt in two runs in a 96-well format.
- Fast synthesis: Oligos are ready for use in 7-13 hours depending on the length of the longest oligo.
- No harsh chemicals: Enzymatic DNA Synthesis (EDS) technology enables automated template-free synthesis without hazardous reagents.
- Application-ready output: Oligos are desalted and normalized in molecular biology grade water and suitable for downstream applications.
- Custom iDNA: Add a leading 5’ sequence of 15-40 nt to all oligos synthesized (maximum total length of 100 nt) using the SYNTAX 60-Cycle Custom iDNA Kit, 96 Oligos (sold separately).
SYNTAX 80-Cycle Kits, 96 Oligos
The SYNTAX 80-Cycle Kits, 96 Oligos include the reagent and consumables required for the parallel enzymatic synthesis of 96 ready-to-use DNA oligos of up to 80 nt in length. Oligos are synthesized rapidly to enable the same or next day use in downstream workflows. Both the synthesis and processing kits are required for oligo synthesis using the SYNTAX System.
- Flexible runs: Synthesize up to 96 oligos of ≤80 nt in one run or up to 384 oligos of ≤20 nt in four runs using the SYNTAX 80-Cycle Synthesis Kit. One SYNTAX 80-Cycle Processing Kit, 96 Oligos is required per run. (All runs must be performed within 7 days.)
- Fast synthesis: Oligos are ready in less than a day with the workflow completed in approximately 7-17 hours depending on oligo length.
- No harsh chemicals: Enzymatic DNA Synthesis (EDS) technology enables automated template-free synthesis without hazardous reagents.
- Application-ready output: Oligos are desalted and normalized in molecular biology grade water and suitable for downstream applications.
- Custom iDNA: Add a leading 5’ DNA sequence of up to 45 nt to all oligos synthesized (maximum total length of 125 nt) using the SYNTAX 80-Cycle Custom iDNA Processing Kit (sold separately).
SYNTAX 192 Label Kit
The SYNTAX 192 Label Kit includes supplemental reagents and consumables required for the parallel enzymatic synthesis of 96 ready-to-use labeled DNA probes on a SYNTAX System. Labeled probes are synthesized rapidly in less than a day, enabling same or next day use in downstream workflows. Probe synthesis requires the SYNTAX 80-Cycle Synthesis Kit, 96 Oligos, the SYNTAX 80-Cycle Processing Kit, 96 Oligos, and SYNTAX Labels (sold separately).
- Rapidly synthesize up to 96 ready-to-use labeled probes in less than a day on a SYNTAX system.
- Use labeled probes for a variety of techniques, including qPCR/dPCR, NGS target enrichment, and FISH.
- Select from a menu of SYNTAX Labels that includes five fluorophores, three quenchers, or biotin. Up to two labels can be incorporated in each probe. (Each sold separately.)
- Enzymatic DNA Synthesis (EDS) technology enables automated template-free synthesis without hazardous reagents.
- Fluorophores and quenchers are available in 16 and 48 reaction sizes. Biotin available in 96 reaction size.
Labeled Probe Guidance
| Fluorophore | Peak Excitation | Peak Emission | Quencher | |
|---|---|---|---|---|
| Green (488) | 495 nm | 519 nm | Quencher 1 | Use with FAM channel |
| Yellow (532) | 532 nm | 552 nm | Quencher 1 | Use with HEX channel |
| Orange (550) | 554 nm | 576 nm | Quencher 2 | Use with TAMRA channel |
| Red (565) | 564 nm | 590 nm | Quencher 2 | Use with ROX channel |
| Deep Red (647) | 646 nm | 664 nm | Quencher 3 | Use with CY5 channel |
Select the Right SYNTAX Kit for Your Application
Synthesizing oligos with the SYNTAX STX-100 System requires specific synthesis kits. With two types of SYNTAX kits available for different applications, consult the table to better select the appropriate kit for your needs.
| SYNTAX 60-Cycle Kit, 96 Oligos | SYNTAX 80-Cycle Kits, 96 Oligos | |
|---|---|---|
| APPLICATIONS | Gene Synthesis, Mutagenesis, CRISPR | q/d/PCR, Sequencing |
| PROBE SYNTHESIS | Not supported | Requires the SYNTAX 192 Label Kit and SYNTAX labels (fluorophores, quenchers, or biotin). (Sold separately.) |
| RUNS PER KIT | 1-2 runs | SYNTAX 80-Cycle Synthesis Kit, 96 Oligos: 1-4 runs SYNTAX 80-Cycle Processing Kit, 96 Oligos: 1 run Sold separately. |
| MAXIMUM OLIGO LENGTH | 60 nt x 96 oligos in one run 30 nt x 192 oligos in two runs | 80 nt x 96 oligos in one run 40 nt x 192 oligos in two runs 27 nt x 288 oligos in three run 20 nt x 384 oligos in four runs |
| YIELD AND CONCENTRATION | Unlabeled oligos: ≥200 pmol and ≥5 μM | Unlabeled oligos: ≥600 pmol and ≥7 μM Probes: ≥400 pmol and ≥5 μM |
| KITS REQUIRED | SYNTAX 60-Cycle Kit, 96 Oligos | SYNTAX 80-Cycle Synthesis Kit, 96 Oligos SYNTAX 80-Cycle Processing Kit, 96 Oligos |
| CUSTOM iDNA | 15-40 nt Requires the SYNTAX 60-Cycle Custom iDNA Kit, 96 Oligos. (Sold Separately.) | 15-45 nt Requires the SYNTAX 80-Cycle Synthesis Kit, 96 Oligos are required for oligo synthesis on the SYNTAX STX-100 system. (Sold Separately.) |
| PACK SIZES | Single kit Pack of 4 SYNTAX 60-Cycle Custom iDNA Kit, 96 Oligos: pack of 4 only | Single kit Pack of 4 SYNTAX 80-Cycle Custom iDNA Processing Kit, 96 Oligos: pack of 8 only |
Primers and probes printed on the SYNTAX System in hours perform comparably to those ordered from service providers.
| Catalog Number | Description |
|---|---|
| 100134 | SYNTAX 60-Cycle Kit, 96 Oligos |
| 100294 | SYNTAX 60-Cycle Kit, 96 Oligos, pack of 4 |
| 100274 | SYNTAX 60-Cycle Custom iDNA Kit, 96 Oligos, pack of 4 |
| 100852 | SYNTAX 80-Cycle Synthesis Kit, 96 Oligos |
| 100853 | SYNTAX 80-Cycle Synthesis Kit, 96 Oligos, pack of 4 |
| 100854 | SYNTAX 80-Cycle Processing Kit, 96 Oligos |
| 100855 | SYNTAX 80-Cycle Processing Kit, 96 Oligos, pack of 4 |
| 100856 | SYNTAX 80-Cycle Custom iDNA Processing Kit, 96 Oligos |
| 100786 | SYNTAX 192 Label Kit |
| 100779 | SYNTAX 48 Label, Green (488) |
| 100934 | SYNTAX 48 Label, Yellow (532) |
| 100932 | SYNTAX 48 Label, Orange (550) |
| 100780 | SYNTAX 48 Label, Red (565) |
| 100781 | SYNTAX 48 Label, Deep Red (647) |
| 100773 | SYNTAX 48 Label, Quencher 1 |
| 100774 | SYNTAX 48 Label, Quencher 2 |
| 100775 | SYNTAX 48 Label, Quencher 3 |
| 100782 | SYNTAX 16 Label, Green (488) |
| 100935 | SYNTAX 16 Label, Yellow (532) |
| 100933 | SYNTAX 16 Label, Orange (550) |
| 100783 | SYNTAX 16 Label, Red (565) |
| 100784 | SYNTAX 16 Label, Deep Red (647) |
| 100776 | SYNTAX 16 Label, Quencher 1 |
| 100777 | SYNTAX 16 Label, Quencher 2 |
| 100778 | SYNTAX 16 Label, Quencher 3 |
| 100785 | SYNTAX 96 Label, Biotin |