Join DNA Script at ABRF 2022
DNA Script is sponsoring the 2022 Association of Biomolecular Resource Facilities (ABRF) Annual Meeting, which will be held in Palm Springs, California from March 27 to March 30. Please join us and our ABRF colleagues in the shared research resources community for this year’s meeting focused on Celebrating Science, Community, Synergy and Resiliency as we connect through engaging sessions and events. The program includes:
More than 40 scientific sessions to choose among
Interactive networking opportunities
Brief Innovation Theater presentations
Technology Showcases featuring ABRF Sponsors
Join DNA Script at the following ABRF activities to learn more about how your core lab can bring the SYNTAX System and the power on DNA on demand to your institution. Sign up to reserve your space at each of the activities below.
Technology Showcase | Mar 29, 7:30 – 8:30am | Smoketree AB
SYNTAX system – Benchtop enzymatic DNA synthesis delivers on-demand DNA and same-day results, presented by Steven Quistad, Ph.D. and Bailey Schmidt
Abstract: This showcase will provide an overview of Enzymatic DNA Synthesis (EDS) and the core technology behind the SYNTAX System, the world’s first benchtop enzymatic DNA printer developed by DNA Script. The SYNTAX system was installed in the Institut Pasteur (Paris, France) in February 2021 during the height of the COVID-19 pandemic. During this period it was particularly difficult for many teams to receive oligos from commercial vendors with some groups waiting as long as 3 months for oligo delivery. The DNA on-demand capabilities of the SYNTAX system were able to relieve the oligo bottleneck created by the COVID-19 pandemic and rapidly move projects forward during a period of severe supply chain uncertainty. The SYNTAX System enabled a variety of applications including custom rRNA depletion, COVID-19-related projects and various mutagenesis studies. In addition, one group was able to leverage the unique iDNA capabilities of the SYNTAX System to reduce library preparation time and reagent costs for Illumina sequencing. Taken together the utility of the SYNTAX System within a core lab environment was demonstrated across a variety of scientific disciplines and directly contributed to the acceleration of data generation.
Morning Eye Opener | Mar 30, 9:30am
Using Enzymatic DNA Synthesis to Build Custom Library Adaptors for Controlling Real-Time Nanopore Sequencing, presented by Tim Mercer, Ph.D., University of Queensland
Abstract: Benchtop enzymatic DNA synthesis enables the rapid creation of oligonucleotides that can be combined with real-time nanopore DNA sequencing to accelerate the iterative design and testing cycles in synthetic biology. Here, we use enzymatic DNA synthesis to build novel, custom library adaptors, termed CAPTORS, that contain functional sequence elements that can measure the qualitative and quantitative accuracy of an DNA sequencing library. We show how CAPTORS are used to prepare libraries from patient samples, with the functional information retrieved in during nanopore sequencing. We show how the analysis of CAPTORS using adaptive sequencing allows real-time evaluation of sequencing accuracy at per-read, -pore and time level during metagenome experiments in the Nevada Toluca volcano near Mexico City. CAPTORS can also analyse quantitative accuracy, improving measurements of gene expression in mRNA samples, and enabling best-in-class normalisation between samples across large patient cohorts, longitudinal patient timelines and point-of-care testing. These benefits can also be employed to improve clinical diagnosis, with CAPTORS able to model and correct systematic sequencing errors to improve the diagnosis of pathogenic BRCA1/2 variants in breast cancer. Together, this demonstrates how the enzymatic synthesis of CAPTORs can affix information within patient samples during library preparation that is later retrieved by sequencing. The information encoded within CAPTORS can act as internal ‘software’ that interfaces with external bioinformatic software for responsive analysis, and integrate patient samples into a clinical IT infrastructure.
Exhibition Booth | March 28-30, 7:30am Open Daily | Booth #220
Visit the DNA Script booth to take a look at and get a demo of the SYNTAX System. There, talk to our team about how we can partner to bring the power of DNA on demand to your core lab.
Networking Event | Monday March 28, 9:00pm | The Village Pub Palm Springs | 266 South Palm Canyon Drive
ABRF Badges Required. You can pick up drink tickets at our booth.