In this Application Note, we demonstrate the use of DNA Script’s Enzymatic DNA Synthesis (EDS) technology and benchtop SYNTAX^TM STX-200 Platform to synthesize oligos for polymerase cycling assembly (PCA) of genes.

Using the 1698bp hemagglutinin (HA) gene as a model, we compared PCA-based assemblies with 60-mer and 120-mer oligos printed using the SYNTAX platform. The HA gene was successfully assembled using both sets of starting oligos.

Incorporating error correction with CorrectASE yielded an 18.5% and 21.9% rate of producing a perfect sequence with 60-mer and 120-mer oligos, meaning fewer than ten clones would have to be picked to obtain at least one perfect, error-free clone.